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Ultrastructural observations of the mature megagametophyte and the fertilization in wheat (Triticum aestivum)

Publication: Canadian Journal of Botany
February 1985

Abstract

The mature embryo sac of wheat contains an egg apparatus composed of an egg cell and two synergids at the micropylar end, a central cell with two large polar nuclei in the middle, and a mass of 20 to 30 antipodals at the chalazal end. A comparison was made of the ultrastructural features of the various cells of the embryo sac. The features included the position of the nucleus and vacuoles, the number, structure, and distribution of organelles, and the extent of the cell walls surrounding each cell. The pollen tube enters one synergid through the filiform apparatus from the micropyle. The penetration and discharge of the pollen tube causes the further degeneration of that synergid, which had already undergone changes before pollination. The second synergid does not change further in appearance following the penetration of the first by the pollen-altered tube. Half an hour after pollination at 20–25 °C, two male nuclei are seen in the cytoplasm of the egg and the central cell. At about 1 h after pollination, one sperm has made contact with the egg nucleus, while the other sperm is fusing with one of the polar nuclei.

Résumé

Le sac embryonnaire mature du blé contient un appareil composé d'une oosphère et deux synergides à l'extrémité micopylaire, une cellule centrale avec deux gros noyaux polaires au milieu et une masse de 20 à 30 antipodes à l'extrémité chalazienne. Nous avons comparé les caractéristiques ultrastructurales des diverses cellules du sac embryonnaire. Les caractères comprenaient la position du noyau et des vacuoles, le nombre, la structure et la distribution des organites, et l'étendue des parois cellulaires entourant chaque cellule. Le tube pollinique pénètre une synergide à travers l'appareil filiforme. La pénétration et la décharge du tube pollinique cause un surcroît de dégénérescence de la synergide en question qui avait déjà subi des changements avant la pollinisation. La deuxième synergide ne change pas d'apparence davantage après la pénétration de la première par le tube pollinique. Une demi-heure après la pollinisation à 20–25 °C, deux noyaux mâles sont visibles dans le cytoplasme de l'oosphère et de la cellule centrale. Une heure après la pollinisation, un gamète mâle est en contact avec le noyau de l'oeuf, tandis que l'autre fusionne avec un des noyaux polaires. [Traduit par le journal]

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cover image Canadian Journal of Botany
Canadian Journal of Botany
Volume 63Number 2February 1985
Pages: 163 - 178

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Version of record online: 31 January 2011

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