Fungal symbionts of endangered Crocanthemum canadense (Cistaceae) in Nova Scotia

Publication: Botany
9 March 2021

Abstract

Crocanthemum canadense (L.) Britton (Cistaceae) is critically imperiled in Nova Scotia. The decline of Nova Scotian Crocanthemum canadense is largely due to the loss of the Annapolis Valley sand barrens habitat. Fungal symbionts may aid in nutrient and water acquisition as well as plant defenses. The role of fungal associations with Crocanthemum canadense is unknown; our goal was to identify fungal symbionts to inform ongoing conservation research. We isolated fungi from eighteen Crocanthemum canadense plants collected from Greenwood, Nova Scotia. Using internal transcribed spacer (ITS) rDNA barcoding of fungal cultures, we identified 58 fungal taxa. ITS2 meta-amplicon barcoding of roots and rhizosphere soil revealed 241 fungi with basidiomycetes accounting for 53.8% of reads. Chaetothyriales sp., Mycetinis scorodonius, Acidomelania panicicola, and Scleroderma citrinum were the most abundant root associates based on meta-amplicon data. We quantified percent root colonization of arbuscular mycorrhizal fungi (AMF) using root staining and microscopy. The average AMF colonization rate of the roots was 29.6% (n = 18). Our research provides a foundation for understanding the fungal community in this declining habitat and the first account of fungal symbionts in the above- and below-ground tissues and rhizosphere of Crocanthemum canadense. Identifying fungi influencing endangered Nova Scotian Crocanthemum canadense is valuable for developing conservation strategies.

Résumé

Crocanthemum canadense (L.) Britton (Cistaceae) est en grand danger d’extinction en Nouvelle-Écosse. Le déclin de Crocanthemum canadense néo-écossais est principalement dû à la perte d’habitat des landes de sable de la vallée de l’Annapolis. Les symbiotes fongiques peuvent contribuer à l’acquisition de nutriments et d’eau ainsi qu’à la défense des plantes. Le rôle des associations fongiques avec Crocanthemum canadense est inconnu ; l’objectif des auteurs consistait à identifier les symbiotes fongiques afin de guider les recherches en cours sur la conservation. Ils ont isolé des champignons à partir de dix-huit plants de Crocanthemum canadense récoltés à Greenwood, en Nouvelle-Écosse. En utilisant le code à barres de l’interne transcrit spacer (ITS) de l’ADNr des cultures fongiques, ils ont identifié 58 taxons fongiques. Le codage à barres du méta-amplicon ITS2 des racines et du sol de la rhizosphère a révélé la présence de 241 champignons, les basidiomycètes représentant 53,8 % des lectures. Chaetothyriales sp., Mycetinis scorodonius, Acidomelania panicola, et Scleroderma citrinum étaient les espèces associées racines les plus abondantes sur la base des données méta-amplicon. Les auteurs ont quantifié le pourcentage de colonisation des racines par les champignons mycorhiziens à arbuscules (CMA) en utilisant la coloration des racines et la microscopie. Le taux moyen de colonisation des racines par les CMA était de 29,6 % (n =18). Ils fournissent une base pour comprendre la communauté fongique dans cet habitat en déclin et la première description des symbiotes fongiques dans les tissus aériens et souterrains et la rhizosphère de Crocanthemum canadense. L’identification des champignons influençant Crocanthemum canadense en Nouvelle-Écosse, une espèce menacée, est précieuse pour l’élaboration de stratégies de conservation. [Traduit par la Rédaction]

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cover image Botany
Botany
Volume 99Number 7July 2021
Pages: 403 - 419

History

Received: 7 October 2020
Accepted: 20 January 2021
Published online: 9 March 2021

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Key Words

  1. AMF
  2. Cistaceae
  3. Crocanthemum
  4. endangered species
  5. endophyte
  6. mycorrhiza
  7. sand barren

Mots-clés

  1. CMA
  2. Cistaceae
  3. Crocanthemum
  4. espèce menacée
  5. endophyte
  6. mycorhize
  7. lande de sable

Authors

Affiliations

Philicity R.M. Byers 130548b@acadiau.ca
Department of Earth and Environmental Science, Faculty of Pure and Applied Science, Acadia University, Wolfville, NS B4P 2R6, Canada.
Rodger C. Evans
Department of Biology, Faculty of Pure and Applied Science, Acadia University, Wolfville, NS B4P 2R6, Canada.
Zoë Migicovsky
Department of Plant, Food and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS B2N 5E3, Canada.
Allison K. Walker
Department of Biology, Faculty of Pure and Applied Science, Acadia University, Wolfville, NS B4P 2R6, Canada.

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