The area and volume of single human erythrocytes during gradual osmotic swelling to hemolysis
Publication: Canadian Journal of Physiology and Pharmacology
June 1970
Abstract
A double-chambered slide was designed for the microscope which would enable continuous viewing of cells hanging on edge in a Ringer solution which was gradually being reduced in osmotic pressure. This was achieved by putting a dialysis membrane between the cell chamber and a chamber containing distilled water. Photographs were taken at 1-min intervals of single cells on edge (revealing the biconcave profile) until the cells hemolyzed, usually within 30 min. The area and volume of revolution of each cell were calculated from measurements on photographic enlargements. No significant change in area occurs during the swelling series although the red cell changes gradually from biconcave to spherical and remains spherical for approximately 7 min before hemolyzing. This stability is best explained by a leakage of potassium ion from the cell prior to hemolysis (which has been reported by Seeman to be approximately 20%).
Résumé
non disponible
Get full access to this article
View all available purchase options and get full access to this article.
Information & Authors
Information
Published In
Canadian Journal of Physiology and Pharmacology
Volume 48 • Number 6 • June 1970
Pages: 369 - 376
History
Version of record online: 12 February 2011
Authors
Metrics & Citations
Metrics
Other Metrics
Citations
Cite As
Peter B. Canham and David R. Parkinson. 1970. The area and volume of single human erythrocytes during gradual osmotic swelling to hemolysis. Canadian Journal of Physiology and Pharmacology.
48(6): 369-376. https://doi.org/10.1139/y70-059
Export Citations
If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.
Cited by
1. Simultaneous observation of shape, volume, and hemoglobin content of a single red blood cell under the osmotic pressure difference
2. Effects of rigidity on tension within the cell membranes of erythrocytes swollen by osmotic shock
3. Dynamic and reversible shape response of red blood cells in synthetic liquid crystals
4. Simulation of the osmosis-based drug encapsulation in erythrocytes
5. Water Efflux in Red Blood Cells of Sickle Cell Patients under Spontaneous Deoxygenation
6. A continuum model of docking of synaptic vesicle to plasma membrane
7. Analysis of Mechanical Behavior of Red Blood Cell Membrane with Malaria Infection
8. An image-processing technique for measuring the dynamic movement of cell membranes
9. Incoherent light transport in an anisotropic random medium: A probe of human erythrocyte aggregation and deformation
10. Stationary deformations of erythrocytes by high-frequency electric field
11. Repetitive cell ‘jumps’ during hypotonic lysis of erythrocytes observed with a simple flow chamber
12. Stereological studies on red corpuscle size produce values different from those obtained using haematocrit‐ and model‐based methods
13. On Red Blood Cells, Hemolysis and Resealed Ghosts
14. Thermal denaturation of the erythrocyte cytoskeleton alters the morphological changes associated with osmotic swelling
15. Measurement of biophysical properties of red blood cells by resistance pulse spectroscopy: volume, shape, surface area, and deformability
16. Temperature effects on osmotic fragility, and the erythrocyte membrane
17. Normal and homogeneous red blood cell populations over a wide range of hyper‐iso‐hypotonic media
18. Effects of glutaraldehyde and critical point drying on the shape and size of erythrocytes in isotonic and hypotonic media
19. Mechanoelastic Properties of Biological Membranes
20. Osmotic hemolysis and fragility A new model based on membrane disruption, and a potential clinical test
21. Edge energy and pore stability in bilayer lipid membranes
22. Transport of Water and Nonelectrolytes Across Red Cell Membranes
23. Relations between membrane monolayers in some red cell shape transformations
24. The Red Cell Shape as an Indicator of Membrane Structure: Ponder’s Rule Reexamined
25. A computerized method for the determination of the osmotic fragility curve of erythrocytes
26. Some clues as to the formation of protrusions by
Fundulus
deep cells1
27. Static equilibrium configurations of a model red blood cell
28. Elastic deformations of red blood cells
29. Permeability of the human erythrocyte to glycerol in 1 and 2 m solutions at 0 or 20 °C
30. A physical basis for red cell membrane elasticity
31. Mechanical properties of cellular membranes
32. Hypotonic hemolysis of human red blood cells: a two-phase process
33. Effects of tris and histidine on human erythrocytes and conditions influencing their mode of action
34. Deformation and haemolysis of red cells in shear flow
35. Hemolysis, Induced by Pulsed Laser Irradiation, Transmitted along Rouleaux of Human Red Blood Cells
36. Anesthetics expand erythrocyte membranes without causing loss of K+
37. Stability of the thin elastic shell model of the red blood cell
38. The rate of sedimentation of individual human red blood cells
39. Appendix D: Mechanical Equations of Membrane Equilibrium
View Options
Get Access
Login options
Check if you access through your login credentials or your institution to get full access on this article.
Subscribe
Click on the button below to subscribe to Canadian Journal of Physiology and Pharmacology