Cyclocarya paliurus prevents high fat diet induced hyperlipidemia and obesity in Sprague–Dawley rats
Publication: Canadian Journal of Physiology and Pharmacology
13 April 2015
Abstract
Cyclocarya paliurus (CP; qing qian liu), which is used as an herbal tea in China, has been confirmed to have therapeutic effects on hyperlipidemia and obesity, and therefore it is widely consumed to prevent metabolic diseases such as hyperlipidemia and diabetes. In this study, we investigated the preventive effects of CP on obesity and hyperlipidemia, as well as the underlying mechanisms involved in intestinal secretion of apolipoprotein (apo) B48. Sprague–Dawley rats were fed a high-fat diet (HFD) and with or without various concentrations of an ethanol extract of CP (CPE; 2, 4, or 8 g·(kg body mass)–1) administered by gavage for 8 weeks. From the results we see that CPE dose-dependently blocked increases in body mass, and decreased food utilization as well as visceral fat mass. Decreased serum levels of total cholesterol, triglycerides, and low density lipoprotein cholesterol, and elevated levels of high density lipoprotein cholesterol, as well as lowered levels of total cholesterol and triglycerides in the liver were also noticed in CPE-treated rats. Magnetic resonance images indicated that the abnormal fat storage induced by the HFD was obviously suppressed by CPE. In addition, ELISA analysis showed reduced fasting serum apoB48 in the CPE treatment groups. Based on the above results, CPE shows a promising preventive effect on obesity and hyperlipidemia, partially through suppressing intestinal apoB48 overproduction.
Résumé
Il a été confirmé que, sous forme de tisane en Chine, Cyclocarya paliurus (CP ; « qing qian liu ») possède des effets thérapeutiques sur l’hyperlipidémie et l’obésité. Par conséquent, cette plante est largement consommée en vue de prévenir des troubles métaboliques comme l’hyperlipidémie et le diabète. Dans le cadre de la présente étude, nous avons tenté d’étudier l’effet préventif de CP sur l’obésité et l’hyperlipidémie, ainsi que les mécanismes sous-jacents reliés à la sécrétion intestinale d’apolipoprotéine B48 (apoB48). Un régime à haute teneur en matières grasses a été administré par gavage à des rats Sprague Dawley pendant 8 semaines, avec ou sans extrait de CP dans l’éthanol (CPE) à diverses concentrations (2, 4 et 8 g·(kg de masse corporelle)–1). Les résultats indiquent que l’administration de CPE a inhibé le gain de poids et réduit l’ingestion de nourriture et la masse de tissu adipeux viscéral, et ce, de façon proportionnelle à la dose. Chez les rats traités par le CPE, nous avons aussi noté une diminution des taux sériques de cholestérol total, de triglycérides et de cholestérol-LDL accompagnée d’une augmentation des taux de cholestérol-HDL ainsi qu’une diminution des taux hépatiques de cholestérol total et de triglycérides. L’imagerie par résonance magnétique (IRM) indiquait que l’accumulation anormale de matières grasses entraînée par le régime à haute teneur en matières grasses était supprimée de façon évidente par l’administration de CPE. De plus, une analyse par ÉLISA a montré une réduction des taux sériques d’apoB48 à jeun dans le groupe CPE. Ces résultats indiquent que l’administration de CPE a un effet préventif prometteur sur l’obésité et l’hyperlipidémie, et ce, en partie par la suppression de la surproduction intestinale d’apoB48. [Traduit par la Rédaction]
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Published In
Canadian Journal of Physiology and Pharmacology
Volume 93 • Number 8 • August 2015
Pages: 677 - 686
History
Received: 26 November 2014
Accepted: 1 April 2015
Accepted manuscript online: 13 April 2015
Version of record online: 13 April 2015
Copyright
© 2015.
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Xiaoming Yao, Zi Lin, Cuihua Jiang, Meng Gao, Qingqing Wang, Nan Yao, Yonglan Ma, Yue Li, Shengzuo Fang, Xulan Shang, Yicheng Ni, Jian Zhang, and Zhiqi Yin. 2015. Cyclocarya paliurus prevents high fat diet induced hyperlipidemia and obesity in Sprague–Dawley rats. Canadian Journal of Physiology and Pharmacology.
93(8): 677-686. https://doi.org/10.1139/cjpp-2014-0477
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