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A library of Solanum lycopersicoides introgression lines in cultivated tomato

Publication: Genome
August 2005

Abstract

A set of introgression lines (ILs), containing individual chromosome segments from the wild nightshade Solanum lycopersicoides bred into the genetic background of cultivated tomato (Lycopersicon esculentum), has been developed. A primary group of 56 lines was selected for maximum representation of the S. lycopersicoides genome (~96% of the total map units), homozygosity, and a minimum number of introgressed segments per line. A secondary set of 34 lines provides increased map resolution in certain regions. Approximately 34% of the lines were sterile in the homozygous condition, but could be maintained by heterozygotes. To facilitate identification of segregating ILs, restriction fragment length polymorphism probes were converted to higher throughput cleaved amplified polymorphic sequence markers, which supplement allozyme and morphological loci. Strong segregation distortion was observed in F2 progeny of heterozygous ILs, with an excess of L. esculentum alleles in most regions. For introgressions on distal chromosome 1L, a preferential transmission of S. lycopersicoides alleles was observed in the male germ line. Homozygous ILs generally yielded less seed from self pollination than corresponding heterozygotes, indicating that sterility effects were recessive. This IL library provides a novel resource for genetic studies of traits found in S. lycopersicoides.Key words: Lycopersicon esculentum, Solanum lycopersicum, Solanum lycopersicoides, segregation distortion, alien introgression.

Résumé

Une collection de lignées d'introgression (IL) contenant des segments chromosomiques individuels provenant de la solanacée sauvage Solanum lycopersicoides introduits chez la tomate cultivée (Lycopersicon esculentum) a été produite. Un groupe 'primaire', comprenant 56 lignées, a été constitué pour maximiser la représentation du génome du S. lycopersicoides (~96 % de la distance génétique) et l'homozygotie tout en minimisant le nombre de segments introduits dans chaque lignée. Un groupe 'secondaire', de 34 lignées, permet d'accroître la résolution de la carte dans certaines régions. Environ 34 % des lignées étaient stériles à l'état homozygote, mais ont été maintenues à l'état hétérozygote. Pour faciliter l'identification des IL en ségrégation, des marqueurs RFLP ont été transformés en marqueurs CAPS, lesquels complémentaient les locus isoenzymatiques et des marqueurs morphologiques. Une grande distorsion de la ségrégation a été observée au sein des F2 de lignées hétérozygotes, les allèles du L. esculentum étant favorisés pour la plupart des régions. Pour les introgressions situées en position distale sur le chromosome 1L, une transmission préférentielle des allèles du S. lycopersicoides a été notée du côté mâle. Les lignées IL homozygotes produisaient généralement moins de graines autofécondées que les hétérozygotes correspondants, ce qui suggère que la stérilité était récessive. Cette collection de lignées IL offre une ressource nouvelle pour des études génétiques des caractères présents chez le S. lycopersicoides.Mots clés : Lycopersicon esculentum, Solanum lycopersicum, Solanum lycopersicoides, distorsion de la ségrégation, introgression de chromatine étrangère.[Traduit par la Rédaction]

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cover image Genome
Genome
Volume 48Number 4August 2005
Pages: 685 - 697

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Version of record online: 15 February 2011

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