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Genomic in situ hybridization in Avena sativa

Publication: Genome
August 1994

Abstract

Genomic fluorescent in situ hybridization was employed in the study of the genome organization and evolution of hexaploid oat (Avena sativa L. cv. Sun II, AACCDD, 2n = 6x = 42). Genomic DNAs from two diploid oat species, Avena strigosa (genomic constitution AsAs, 2n = 14) and Avena pilosa (genomic constitution CpCp, 2n = 14), were used as probes in the study. The DNA from A. strigosa labelled 28 of the 42 (2/3) chromosomes of the hexaploid oat, while 14 of the 42 (1/3) chromosomes were labelled with A. pilosa DNA, indicating a close relationship between the A and D genomes. Results also suggested that at least 18 chromosomes (9 pairs) were involved in intergenomic interchanges between the A and C genomes.Key words: oats, Avena sativa L., in situ hybridization, fluorescein isothiocyanate, genome organization.

Résumé

La fluorescence d'hybridation génomique in situ a été utilisée pour l'étude de l'organisation du génome et de l'évolution de l'avoine hexaploïde, l'Avena sativa L. cv. Sun II (AACCDD, 2n = 6x = 42). Les ADN génomiques de deux espèces d'avoines diploïdes, l'Avena strigosa (AsAs, 2n = 14) et l'Avena pilosa (CpCp, 2n = 14), ont servi de sondes dans cette étude. L'ADN de l'A. strigosa a marqué 28 (2/3) des 42 chromosomes de l'avoine hexaploïde, tandis que l'ADN de l'A. pilosa n'en a marqué que 14(1/3), ce qui indique une étroite relation entre les génomes A et D. Les résultats suggèrent également qu'au moins 18 chromosomes (9 paires) ont été impliqués dans les échanges intergénomiques des génomes A et C. Mots clés : avoine, Avena sativa, hybridation in situ, isothiocyanate de fluorescéine, organisation génomique.[Traduit par la Rédaction]

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cover image Genome
Genome
Volume 37Number 4August 1994
Pages: 607 - 612

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Version of record online: 15 February 2011

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