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Genomic in situ hybridization in Avena sativa

Publication: Genome
August 1994


Genomic fluorescent in situ hybridization was employed in the study of the genome organization and evolution of hexaploid oat (Avena sativa L. cv. Sun II, AACCDD, 2n = 6x = 42). Genomic DNAs from two diploid oat species, Avena strigosa (genomic constitution AsAs, 2n = 14) and Avena pilosa (genomic constitution CpCp, 2n = 14), were used as probes in the study. The DNA from A. strigosa labelled 28 of the 42 (2/3) chromosomes of the hexaploid oat, while 14 of the 42 (1/3) chromosomes were labelled with A. pilosa DNA, indicating a close relationship between the A and D genomes. Results also suggested that at least 18 chromosomes (9 pairs) were involved in intergenomic interchanges between the A and C genomes.Key words: oats, Avena sativa L., in situ hybridization, fluorescein isothiocyanate, genome organization.


La fluorescence d'hybridation génomique in situ a été utilisée pour l'étude de l'organisation du génome et de l'évolution de l'avoine hexaploïde, l'Avena sativa L. cv. Sun II (AACCDD, 2n = 6x = 42). Les ADN génomiques de deux espèces d'avoines diploïdes, l'Avena strigosa (AsAs, 2n = 14) et l'Avena pilosa (CpCp, 2n = 14), ont servi de sondes dans cette étude. L'ADN de l'A. strigosa a marqué 28 (2/3) des 42 chromosomes de l'avoine hexaploïde, tandis que l'ADN de l'A. pilosa n'en a marqué que 14(1/3), ce qui indique une étroite relation entre les génomes A et D. Les résultats suggèrent également qu'au moins 18 chromosomes (9 paires) ont été impliqués dans les échanges intergénomiques des génomes A et C. Mots clés : avoine, Avena sativa, hybridation in situ, isothiocyanate de fluorescéine, organisation génomique.[Traduit par la Rédaction]

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cover image Genome
Volume 37Number 4August 1994
Pages: 607 - 612


Version of record online: 15 February 2011


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