Characterization of Bacteroides gingivalis by direct fluorescent antibody staining and cellular fatty acid profiles

Publication: Canadian Journal of MicrobiologyApril 1982https://doi.org/10.1139/m82-056

Abstract

Bacteroides gingivalis is a newly proposed species which includes strains isolated from the mouth. Thirteen strains of B. gingivalis isolated from three geographic locations in the United States and France were examined with direct fluorescent antibody staining and analysis of total cellular fatty acids and compared with 16 strains of B. asaccharolyticus of nonoral origin by the same methods. Bacteroides gingivalis strains reacted with the B. gingivalis conjugate (fluorescein isothiocyanate labeled antibody reagent) only, while the B. asaccharolyticus strains reacted with the B. asaccharolyticus conjugate only. The B. gingivalis strains showed negative fluorescence with fluorescein isothiocyanate conjugates for other black-pigmented Bacteroides species. The specificity of the B. gingivalis conjugate was demonstrated by its failure to stain 88 strains of aerobic and anaerobic bacteria other than B. gingivalis. The fatty acid profiles of B. gingivalis and B. asaccharolyticus were readily distinguishable. The B. gingivalis profile was also distinguishable from those of other pigmenting Bacteroides species on the basis of concentration ratios among the characteristic components. These results support the species separation of B. gingivalis and B. asaccharolyticus. Further, they indicate the usefulness of cellular fatty acid profiles as an adjunct to the use of specific fluorescent antibody conjugates for identification of Bacteroides species.

Résumé

Bacteroides gingivalis est une espèce qui a été proposée récemment et qui comprend des souches isolées de la bouche. Treize souches de B. gingivalis, isolées de trois régions géographiques des États-Unis et de la France, ont été examinées par immunofluorescence directe et par analyse du contenu total des acides gras cellulaires. Les valeurs obtenues ont été comparées par les mêmes méthodes avec 16 souches de B. asaccharolyticus d'origine non-orale. Les souches de B. gingivalis réagissent uniquement avec le sérum fluorescent préparé contre B. gingivalis (anticorps marqués à l'isothiocyanate de fluorescéine) alors que les souches de B. asaccharolyticus réagissent uniquement avec le sérum anti-B. asaccharolyticus. Les souches de B. gingivalis ne montrent pas de réaction de fluorescence positive avec des sérums fluorescents préparés contre d'autres espèces de Bacteroides pigmentées en noir. La spécificité du sérum anti-B. gingivalis a été confirmée par l'absence de réactions croisées avec 88 souches de bactéries aérobies ou anaérobies autres que B. gingivalis. Les profils d'acides gras de B. gingivalis et de B. asaccharolyticus sont facilement distinguables. Le profil de B. gingivalis est aussi distinguable de ceux de d'autres espèces pigmentées de Bacteroides sur la base des rapports de concentration des composés caractéristiques. Ces résultats appuient la distinction d'espèce entre B. gingivalis et B. asaccharolyticus. De plus, ils confirment l'utilité des profils de mesure des acides gras cellulaires comme outil complémentaire à l'utilisation d'antisérums fluorescents spécifiques pour l'identification des espèces de Bacteroides.[Traduit par le journal]
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Canadian Journal of Microbiology cover image
Canadian Journal of Microbiology
Volume 28Number 4April 1982
Pages: 367 - 374

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Published online: 10 February 2011

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